Reporter

Part:BBa_K2429017

Designed by: Tinna-Solveig F Kosoko-Thoroddsen   Group: iGEM17_MIT   (2017-10-22)
Revision as of 21:03, 23 October 2017 by Nmyrie (Talk | contribs)


2 Exon mKate-HBG Reporter

This part splits the red fluorescent mKate reporter into two exons, with human beta globin (HBG) intron 2 in between these two exons. We used this split mKate 2-exon construct as a reporter construct that we used to determine if our dCas13 or Ms2 systems were successful in splicing out the HBG Intron 2. We used HBG Intron 2 because it is a standard intron used in reporter constructs.

<img src="2ex.png" alt="2 Exon reporter">

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1582
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1487
    Illegal SapI.rc site found at 55


[edit]
Categories
//cds/reporter
//function/reporter/fluorescence
Parameters
biology
protein