Composite

Part:BBa_K2332003:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-09-29)
Revision as of 00:22, 23 October 2017 by Paola handal (Talk | contribs) (→‎References)


tRNA-Pyl (pylT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

E. coli cells must be amberless in order to test the pyrrolysine incorporation only in the desired proteins.


Source

Sequence obtained from: BBa_K1223014

References

1. Mitra N. Incorporating Unnatural Amino Acids into Recombinant Proteins in Living Cells. Materials and Methods. 2013;3.

2. Wang Y, Wu B, Wang Z, Huang Y, Wan W, Russell W et al. A genetically encoded photocaged Nε-methyl-l-lysine. Molecular BioSystems. 2010;6(9):1557.