DNA

Part:BBa_K2429009

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-12)
Revision as of 01:37, 13 October 2017 by Registry (Talk | contribs)

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pENTR L. shahii dCas13a-DDX6

Leptotrichia shahii deactivated Cas13a-DDX6 protein coding region. This part produces a deactivated version of the CRISPR protein Cas13a, which normally binds and cuts mRNA. With the deactivation sequence, the protein becomes catalytically inactive, and unable to cut the mRNA; however, it can still bind to the mRNA molecule.Linked to the sequence coding for the protein is a sequence coding for DDX6 domain. This added domain prohibits the initiation of RNA translation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5835
    Illegal PstI site found at 1867
    Illegal PstI site found at 2236
    Illegal PstI site found at 2965
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5835
    Illegal PstI site found at 1867
    Illegal PstI site found at 2236
    Illegal PstI site found at 2965
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5835
    Illegal BglII site found at 611
    Illegal BglII site found at 1235
    Illegal BglII site found at 1631
    Illegal BglII site found at 1922
    Illegal BglII site found at 2012
    Illegal BglII site found at 3173
    Illegal BglII site found at 3260
    Illegal BamHI site found at 1
    Illegal BamHI site found at 4255
    Illegal BamHI site found at 4357
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5835
    Illegal PstI site found at 1867
    Illegal PstI site found at 2236
    Illegal PstI site found at 2965
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5835
    Illegal PstI site found at 1867
    Illegal PstI site found at 2236
    Illegal PstI site found at 2965
    Illegal NgoMIV site found at 4212
    Illegal NgoMIV site found at 4231
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 597
    Illegal SapI.rc site found at 703
    Illegal SapI.rc site found at 1573
    Illegal SapI.rc site found at 2407
    Illegal SapI.rc site found at 5713


[edit]
Categories
//cds
Parameters
biology
protein