DNA

Part:BBa_K2267001

Designed by: HE, BOTAO   Group: iGEM17_TUST_China   (2017-10-21)
Revision as of 14:31, 23 October 2017 by UndertoneTao (Talk | contribs)


A gene encodes glutaminase 1;Catalyze a reaction converting glutamine to glutamate and releasing the

GlsA

GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH.T--TUST China--parts GlsA.gif

Expressed

T--TUST China--parts GlsA pcr.png
Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 967
    Illegal SpeI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 967
    Illegal SpeI site found at 2
    Illegal NotI site found at 9
    Illegal NotI site found at 959
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 967
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 967
    Illegal SpeI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 967
    Illegal SpeI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None