Coding
Part:BBa_K2244003
Designed by: Chen Hong Group: iGEM17_SSTi-SZGD (2017-10-18)
TorA-opdA
This part is a functional gene that encodes organophosphate hydrolase (OPH) to degrade organophosphate pesticides. In order to export hydrolase to the periplasmic space of E.coli , We fused a TorA signal peptide to the N-terminal of opdA (BBa_K215090) that facilitates the exportation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1123
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 220
Illegal AgeI site found at 415
Illegal AgeI site found at 754 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |