Coding
Part:BBa_K2332021:Design
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-18)
Blue light repressible bioluminescence (Pblrep LuxCDABE )
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2621
Illegal AgeI site found at 2888
Illegal AgeI site found at 3416
Illegal AgeI site found at 3997
Illegal AgeI site found at 4444
Illegal AgeI site found at 5322
Illegal AgeI site found at 5537 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Vibrio fischeri regulatory protein LuxR (luxR) gene, complete cds; LuxICDABEG operon sequences were obtained from Genbank: AF170104.1, from which LuxC,D,A,B,E genes were identified and codon optimized for E. coli. Pblrep was designed by Jayaraman P. et al. (2016).
Source
The 5kb construct is too large to obtain as a single construct so it was split into gBlock fragments and each were designed to have 40bp overlaps to the adjacent fragment for gibson assembly.