Coding

Part:BBa_K2332021:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-18)
Revision as of 23:26, 18 October 2017 by Registry (Talk | contribs)

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Blue light repressible bioluminescence (Pblrep LuxCDABE )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2621
    Illegal AgeI site found at 2888
    Illegal AgeI site found at 3416
    Illegal AgeI site found at 3997
    Illegal AgeI site found at 4444
    Illegal AgeI site found at 5322
    Illegal AgeI site found at 5537
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Vibrio fischeri LuxR and Lux operon GenBank: AF170104.1 from which LuxC, D, A, B, E genes were identified and codon optimized for E. coli. The 5kb construct was split into 8 gblock fragments (700-900bp each) and ordered from IDT


Source

The 5kb construct is too large to obtain as a single construct so it was split into gBlock fragments and each were designed to have 40bp overlaps to the adjacent fragment for gibson assembly.


References