Composite

Part:BBa_K2332025:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-20)
Revision as of 18:48, 20 October 2017 by Registry (Talk | contribs)

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Intimin'-mutSpyCatcher (Lys31X, for photocaging)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 880
    Illegal NgoMIV site found at 1621
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amberless E. coli must be used to see the effects of photocageing spyCatcher



Source

SpyCatcher: BBa_K1159200


References