Composite
Part:BBa_K2332025:Design
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-20)
Intimin'-mutSpyCatcher (Lys31X, for photocaging)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 880
Illegal NgoMIV site found at 1621 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Amberless E. coli must be used to see the effects of photocageing spyCatcher
Source
SpyCatcher: BBa_K1159200