Composite

Part:BBa_K2332042:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-20)
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GFP-mutSpyCatcher (Lys31X, for photocaging)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 723


Design Notes

Amberless E. coli must be used to see the effects of photocageing spyCatcher. This construct was designed to not contain any other amber stop codon but at position 31 (Lys31X)


Source

SpyCatcher: BBa_K1159200 GFPmut 3b: Part:BBa_E0040


References