Reporter

Part:BBa_K2272008:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-22)
Revision as of 23:56, 22 October 2017 by Registry (Talk | contribs)

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pBAD P.D. TAT-RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 171
    Illegal NgoMIV site found at 219
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 240
    Illegal AgeI site found at 865
    Illegal AgeI site found at 977
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was desgined to validate the function of the signal peptide.


Source

The GFP coding sequence was from the iGEM 2017 distribution kit.

References