Composite

Part:BBa_K2332054

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-21)
Revision as of 15:33, 21 October 2017 by Registry (Talk | contribs)

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GFP-SpyCatcher (constitutive)

This construct is the fusion of GFP with SpyCatcher under the control of a constitutive promoter. This construct allows to directly observe through fluorescence microscopy its localization, and most importantly its co-localization with SpyTag variants. Particularly for our project, it allows us to quantify its binding onto cell surface displayed Intimin'-SpyTag. To use the GFP-SpyCatcher, one needs to lyse recombinant E. coli cells and purify the protein extract in order to visualize its localization on the cell surface of cells expressing intimin'-SpyTag variants.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 723


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