Composite
Part:BBa_K2332053:Design
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-21)
Blue light inducible expression of GFP-SpyTag (Pblind GFP-SpyTag)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 723
Design Notes
We removed the stop codon in GFP and placed the SpyTag sequence after a linker sequence.
Source
SpyTag sequence was obtained from BBa_K1159201 and GFP from: BBa_E0040. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)