Composite
Part:BBa_K2332053:Design
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-21)
Blue light inducible expression of GFP-SpyTag (Pblind GFP-SpyTag)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 723
Design Notes
To obtain the fusion protein of GFP-SpyTag, we had to remove the stop codon of GFP. Parts were already codon optimized for E. Coli.
Source
https://parts.igem.org/Part:BBa_K1159201 was the source of the SpyTag sequence https://parts.igem.org/Part:BBa_E0040 was for the GFP