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Composite
Part:BBa_K2332052
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-21)
Intimin'-GFP-SpyTag (constitutive)
This construct displays GFP fused with SpyTag on the cell surface of E. coli cells constitutively. Intimin' is a truncated version of Intimin (EaeA intimin) which has shown to display proteins of similar size to GFP fused to its N terminal. This construct allows to directly observe through fluorescence microscopy the functionality of Intimin for cell surface display.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 2826
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 2826 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 2826
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 2826
Illegal NgoMIV site found at 862
Illegal NgoMIV site found at 1603 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2697
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Categories
Parameters
None |