Composite

Part:BBa_K2332052

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-21)
Revision as of 15:33, 21 October 2017 by Registry (Talk | contribs)

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Intimin'-GFP-SpyTag (constitutive)

This construct displays GFP fused with SpyTag on the cell surface of E. coli cells constitutively. Intimin' is a truncated version of Intimin (EaeA intimin) which has shown to display proteins of similar size to GFP fused to its N terminal. This construct allows to directly observe through fluorescence microscopy the functionality of Intimin for cell surface display.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2826
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 2826
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2826
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2826
    Illegal NgoMIV site found at 862
    Illegal NgoMIV site found at 1603
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2697


[edit]
Categories
Parameters
None