![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2332051
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-21)
Intimin'-SpyCatcher (constitutive)
This biobrick is for the constitutive expression of Intimin'-SpyCatcher as part of or bacterial cell aggregation system. This biobrick is the fusion of a truncated version of Intimin and SpyCatcher for the cell surface display of SpyCatcher under the control of of a constitutive promoter. When cells expressing this construct are mixed with cells expressing Intimin'-SpyTag aggregation is expected to occur. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) are binding partners that originate from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 880
Illegal NgoMIV site found at 1621 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |