Coding

Part:BBa_K2332020:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-18)
Revision as of 23:19, 22 October 2017 by Paola handal (Talk | contribs) (→‎Source)


LuxCDABE (E. coli bioluminescence)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2562
    Illegal AgeI site found at 2829
    Illegal AgeI site found at 3357
    Illegal AgeI site found at 3938
    Illegal AgeI site found at 4385
    Illegal AgeI site found at 5263
    Illegal AgeI site found at 5478
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Vibrio fischeri regulatory protein LuxR (luxR) gene, complete cds; LuxICDABEG operon sequences were obtained from Genbank: AF170104.1, from which LuxC,D,A,B,E genes were identified and codon optimized for E. coli.

Source

Vibrio fischeri Genbank: AF170104.1, codon optimized for E. coli. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References

Chong G, Kimyon Ö, Manefield M. Quorum Sensing Signal Synthesis May Represent a Selective Advantage Independent of Its Role in Regulation of Bioluminescence in Vibrio fischeri. PLoS ONE. 2013;8(6):e67443.