Coding

Part:BBa_K2332031

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-19)
Revision as of 20:06, 19 October 2017 by Registry (Talk | contribs)

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GFP-SpyCatcher

This construct is the fusion of GFP with SpyCatcher. This construct allows to directly observe through fluorescence microscopy its localization, and most importantly its co-localization with SpyTag variants. Particularly for our project, it allows us to quantify its binding onto cell surface displayed Intimin'-SpyTag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


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Categories
Parameters
None