Coding
Part:BBa_K2332030
Designed by: Paola Handal Group: iGEM17_UCL (2017-10-19)
Intimin'-GFP-SpyTag
This construct displays GFP fused with SpyTag on the cell surface of E. coli cells. Intimin' is a truncated version of Intimin (EaeA intimin) which has shown to display proteins of similar size to GFP fused to its N terminal. This construct allows to directly observe through fluorescence microscopy the functionality of Intimin for cell surface display.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 801
Illegal NgoMIV site found at 1542 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2636
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Categories
Parameters
None |