Coding

Part:BBa_K2332004:Design

Designed by: Paola Handal   Group: iGEM17_UCL   (2017-10-12)
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EL222, photosensitive DNA-binding protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 81
    Illegal AgeI site found at 306
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to reverse translate and codon optimize the protein sequence for its usage in E. coli and ensure no illegal sites were present.


Source

The reverse translation of EL222 (UniProtKB/Swiss-Prot: Q2NB98.1) optimized for E. coli was obtained as a synthetic gBlock along with the promoter, RBS and terminator sequences.


References