Device

Part:BBa_K2463000:Design

Designed by: Jack Lawrence, Balint Vidos   Group: iGEM17_Warwick   (2017-10-22)
Revision as of 19:07, 22 October 2017 by Registry (Talk | contribs)

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Biofilm and Motility Regulation Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1806
    Illegal BamHI site found at 32
    Illegal BamHI site found at 1190
    Illegal XhoI site found at 1768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
    Illegal NgoMIV site found at 595
    Illegal AgeI site found at 931
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 105


Design Notes

Lacks a degrading tag fused within yhjH gene due to unpredictable interactions.


Source

PLeD Source: www.uniprot.org/uniprot/Q9A5I5 yhjH Source: www.uniprot.org/uniprot/P37646

iGEM registry entries regarding parts: BBa_R0010 -lac BBa_B0034 -rbs BBa_K2012002 -PLeD BBa_B0034 -rbs BBa_C0072 -mnt repressor BBa_B1006 -Terminator BBa_R0073 -mnt promoter BBa_K861090 -Yhjh BBa_B1003 -Second Terminator

References