Composite

Part:BBa_K2350025:Design

Designed by: JO-NING HUNG   Group: iGEM17_NYMU-Taipei   (2017-10-22)
Revision as of 13:57, 22 October 2017 by Registry (Talk | contribs)

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pPIGBACK-PrbcL-IndC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 5564
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1641
    Illegal BglII site found at 6126
    Illegal BamHI site found at 6423
    Illegal XhoI site found at 5361
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6344
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5215
    Illegal BsaI site found at 6026
    Illegal SapI.rc site found at 2904


Design Notes

All Pst1 cutting sites in pPIGBACK-PrbcL-IndC are site-mutated using site-directed mutagnesis.


Source

IndC comes from BBa_K1152008, and pPIGBACK is one of our submitted parts, BBa_K2350009.

References