Composite

Part:BBa_K2350021

Designed by: YA-XUAN YANG   Group: iGEM17_NYMU-Taipei   (2017-10-22)
Revision as of 08:10, 22 October 2017 by Yang20051 (Talk | contribs)


R0010-B0034-Holin-B0010-B0012-J23106-B0034-Endolysin-B0010-B0012-J23118-B0034-NrtA-B0015

Endolysin-Holin-NrtA

Promoter, RBS, and Terminator

　　For more details of the connected promoter, RBS, and terminator, you can see in BBa_K2350018 for endolysin part, BBa_K2350019 for holin part, and BBa_2350004 for NrtA part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1035
    Illegal NheI site found at 1058
    Illegal NheI site found at 1757
    Illegal NheI site found at 1780
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2990
    Illegal BamHI site found at 2360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1362
    Illegal AgeI site found at 1432
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None