Composite

Part:BBa_K2302008

Designed by: Hongyang Wei   Group: iGEM17_NEFU_China   (2017-10-19)
Revision as of 02:14, 20 October 2017 by Registry (Talk | contribs)

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PelB+CALB+His

it contains three enzymes of the serine synthesis pathway. Bacteria harboring this plasmidcan be induced by arabinose to synthesize and secrete serine. A SD sequence (RBS) were added to the front of each coding sequence of serA, serB and serC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1023
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 25
    Illegal NgoMIV site found at 51
    Illegal NgoMIV site found at 744
    Illegal NgoMIV site found at 854
    Illegal AgeI site found at 546
    Illegal AgeI site found at 804
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None