Coding
Part:BBa_K2450251:Design
Designed by: Zoe Ford Group: iGEM17_Oxford (2017-10-21)
TetR CFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We decided to have a C-terminal CFP tag so it would not interfere with the N-terminal DNA binding domain of the TetR.
Source
BBa_K106669 TEV cleavage sequence from Merck CFP from AddGene