Coding

Part:BBa_K2450251:Design

Designed by: Zoe Ford   Group: iGEM17_Oxford   (2017-10-21)
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TetR CFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to have a C-terminal CFP tag so it would not interfere with the N-terminal DNA binding domain of the TetR.


Source

BBa_K106669 TEV cleavage sequence from Merck CFP from AddGene

References