Composite
Part:BBa_K2350012:Design
Designed by: JO-NING HUNG Group: iGEM17_NYMU-Taipei (2017-10-21)
PrbcL-IndC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1641
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2904
Design Notes
All Pst1 cutting sites in PrbcL were site-directed mutated.
Source
IndC is from iGEM distribution kit BBa_K1152008, and PrbcL is from Synechoccocus elongatus PCC7942 genomic DNA.