Composite

Part:BBa_K2350016:Design

Designed by: JO-NING HUNG   Group: iGEM17_NYMU-Taipei   (2017-10-21)
Revision as of 13:38, 21 October 2017 by Lilyhung (Talk | contribs)


BBa_B0015 and Ampicillin resistance gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 950


Design Notes

To insert AmpR-B0015 with EcoR1 and Pst1 cutting sites, we use site-directed mutagenesis to remove Pst1 cutting sites in AmpR nucleotide sequence.


Source

BBa_B0015 is from iGEM distribution kit, and Ampicillin resistance gene is from pBR322 nucleotide sequence.

References

Pei-Hong Chen, Hsien-Lin Liu, Yin-Ju Chen, Yi-Hsiang Cheng, Wei-Ling Lin, Chien-Hung Yeh and Chuan-Hsiung Chang (2012). Enhancing CO2 bio-mitigation by genetic engineering of cyanobacteria