Plasmid_Backbone
pPIGBACK

Part:BBa_K2350009:Design

Designed by: JO-NING HUNG   Group: iGEM17_NYMU-Taipei   (2017-10-21)
Revision as of 08:51, 21 October 2017 by Registry (Talk | contribs)

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Pigment double-crossover homologous gene recombination bacbone (pPIGBACK)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1
    Illegal suffix found at 3880
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 3881
    Illegal PstI site found at 3895
    Illegal NotI site found at 7
    Illegal NotI site found at 1321
    Illegal NotI site found at 3888
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal BglII site found at 1883
    Illegal BamHI site found at 2180
    Illegal XhoI site found at 1118
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1
    Illegal suffix found at 3881
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal XbaI site found at 16
    Illegal SpeI site found at 3881
    Illegal PstI site found at 3895
    Illegal NgoMIV site found at 2101
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 972
    Illegal BsaI site found at 1783


Design Notes

We use site-directed mutagenesis to remove all Pst1 cutting sites in pPIGBACK.



Source

Synechoccocus elongatus PCC7942 genomic DNA, pBR322

References