DNA

Part:BBa_K2350005:Design

Designed by: JO-NING HUNG   Group: iGEM17_NYMU-Taipei   (2017-10-21)
Revision as of 06:50, 21 October 2017 by Registry (Talk | contribs)

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3’-ends of the neutral site II (NSII)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 198
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 760
    Illegal BamHI site found at 1057
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 978
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 660


Design Notes

All Pst1 cutting sites in 3NSII were site-directed mutated.


Source

Synechoccocus elongatus PCC7942 genomic DNA

References