Coding

Part:BBa_K2380005:Design

Designed by: Feodor Belov, Isabelle Feinauer, Sophia Hein, Tim Maier, Patrick Müller, Bea-Marie Spiekermann, Lara Steinel, Stefan Zens   Group: iGEM17_TU_Darmstadt   (2017-10-19)
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Chitinase A1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 150
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 256
    Illegal AgeI site found at 952
    Illegal AgeI site found at 1240
    Illegal AgeI site found at 1303
    Illegal AgeI site found at 1366
    Illegal AgeI site found at 1696
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Protein sequence was retranslated, codom optimized by IDT online tool and all BioBrick relevant cleavagesites were removed


Source

protein sequence from uniprot (P20533). Three chitinase genes (chiA, chiC and chiD) comprise the chitinase system of Bacillus circulans WL-12, Alam et al


References