![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K2230015
Designed by: Yi-Lun Huang Group: iGEM17_Mingdao (2017-10-20)
Pcar-wRBS-PhlF-T-Pr-sRBS-GFP-wRBS-lysis/pSB1C3
Cloning: wRBS-lysis was amplified from Lysis gene (BBa_K117000) using a primer with weak RBS sequence (B0032) and assembled with Pcar-wRBS-PhlF-T-Pr-sRBS-GFP/pSB1C3 (BBa_K2230012)
Function: Suicide genes of lysis was driven under the PhlF-repressed promoter (Pr). And the expression of the repressor (PhlF) would be induced upon glucose by a glucose responsive promoter (Pcar). That is, the bacteria can be killed by the suicide genes in the absence of (or running out of) glucose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1501
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Categories
Parameters
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