Coding

Part:BBa_K2201411:Design

Designed by: Laura Schlueter   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)
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Tyrosyl synthetase library plasmid from M. jannashii with mRFP as optical controle for a selection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1275
    Illegal AgeI site found at 1387
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ttttaaaaaactaacagttgtcagcctgtcccgctttaatatcatacgccgttatacgttgtttacgctttgaggaatcccatatggacgaatttgaaatgataaagagaaacacatctgaaattatcagcgaggaagagttaagagaggttttaaaaaaagatgaaaaatctgctcgtataggttttgaaccaagtggtaaaatacatttagggcattatctccaaataaaaaagatgattgatttacaaaatgctggatttgatataattatattgttggctgatttactggcctatttaaaccagaaaggagagttggatgagattagaaaaataggagattataacaaaaaagtttttgaagcaatggggttaaaggcaaaatatgtttatggaagtgagttccagcttgataaggattatacactgaatgtctatagattggctttaaaaactaccttaaaaagagcaagaaggagtatggaacttatagcaagagaggatgaaaatccaaaggttgctgaagttatctatccaataatgatggttaatggtctgcattatcatggcgttgatgttgctgttggagggatggagcagagaaaaatacacatgttagcaagggagcttttaccaaaaaaggttgtttgtattcacaaccctgtcttaacgggtttggatggagaaggaaagatgagttcttcaaaagggaattttatagctgttgatgactctccagaagagattagggctaagataaagaaagcatactgcccagctggagttgttgaaggaaatccaataatggagatagctaaatacttccttgaatatcctttaaccataaaaaggccagaaaaatttggtggagatttgacagttaatagctatgaggagttagagagtttatttaaaaataaggaattgcatccaatgcgcttaaaaaatgctgtagctgaagaacttataaagattttagagccaattagaaagagattataa


Source

BBa_J04450 BBa_K2201400

References