Part:BBa_K2514000:Design
pSB1C3-miR-21-sponge-6s
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Pervious studies indicated sponges containing bulged sites that are mispaired opposite miRNA are more effective than perfectly complementary sponges for individual microRNAs. And different miR-21 sponges contained different binding sites may have different accuracy for measuring the expression of miR-21 in colorectal cancer cells. So we desinged two different mir-21 sponges with different binding sits to test the efficiency of sponges. One mir-21 sponge contains six miR-21 binding sites with 3-nt spacers for bulged sites. We wanted to compare the accuracy and the effectiveness of different miR-21 sponges contained different binding sites for measuring the expression of miR-21 in colorectal cancer cells.
Source
We designed mir-21 sponges contains six miR-21 binding sites with 3-nt spacers for bulged sites based on the sequence of hsa-miR-21 according to the previous study (Ebert MS, Neilson JR, Sharp PA. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods. 2007 Sep;4(9):721-6)
References
Ebert MS, Neilson JR, Sharp PA. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods. 2007 Sep;4(9):721-6 Ebert MS, Sharp PA. MicroRNA sponges: progress and possibilities. RNA. 2010 Nov;16(11):2043-50.