Part:BBa_K2259010
Rop protein - global copy number inhibitor (SynORI framework)
Rop (also known as repressor of primer) is a small protein responsible for keeping the copy number of ColE1 and related bacterial plasmids low in E. coli. Inhibits plasmid replication of all SynORI framework plasmid groups non-specifically, also other ColE1 replicon plasmids.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Contents
Introduction
Biology
Repressor of primer (ROP) is a small dimeric protein that participates in ColE1 plasmid family copy number control, by increasing affinity between two complementary RNAs - RNA I (Replication inhibitor) and RNA II (Replication activator). [1] By increasing affinity of the two RNA molecules Rop decreases the rate of of plasmid replication initiation events.
Rop dimer is a bundle of four tightly packed alpha helices that are held by hydrophobic interactions (Fig. 1).
Usage with SynORI (Framework for multi-plasmid systems)
Characterization of Rop protein (Vilnius-Lithuania 2017)
References
- ↑ Castagnoli L, Scarpa M, Kokkinidis M, Banner DW, Tsernoglou D, Cesareni G. Genetic and structural analysis of the ColE1 Rop (Rom) protein. The EMBO Journal. 1989;8(2):621-629.
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