Coding

Part:BBa_K2259010

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-01)
Revision as of 23:15, 18 October 2017 by LaurynasK (Talk | contribs) (→‎Biology)


Rop protein - global copy number inhibitor (SynORI framework)

Rop (also known as repressor of primer) is a small protein responsible for keeping the copy number of ColE1 and related bacterial plasmids low in E. coli. Inhibits plasmid replication of all SynORI framework plasmid groups non-specifically, also other ColE1 replicon plasmids.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Formatting test Figure 1: Plasmid map of the plasmid p68 used for the characterization of PalcA.

Biology

Repressor of primer (ROP) is a small dimeric protein that participates in ColE1 plasmid family copy number contro, by increasing affinity between two complementary RNAs - RNA I (Replication inhibitor) and RNA II (Replication activator). [1]

Rop dimer is a bundle of four tightly packed alpha helices that are held by hydrophobic interactions.

Usage in SynORI system

  1. ↑ Castagnoli L, Scarpa M, Kokkinidis M, Banner DW, Tsernoglou D, Cesareni G. Genetic and structural analysis of the ColE1 Rop (Rom) protein. The EMBO Journal. 1989;8(2):621-629.
[edit]
Categories
//awards/part_collection/2017
//collections/synori
Parameters
None