Composite

Part:BBa_K2443011

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-18)
Revision as of 14:54, 18 October 2017 by Registry (Talk | contribs)

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LeuRS optimized for expression in E. coli

Leucine tRNA synthetase responsible for attaching leucine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 1534
    Illegal AgeI site found at 1004
    Illegal AgeI site found at 1031
    Illegal AgeI site found at 1085
    Illegal AgeI site found at 1162
    Illegal AgeI site found at 1934
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/cell-free_tx-tl
Parameters
None