Part:BBa_K2278022:Design
Lecrocin I antimicrobial peptide with Alpha-Factor Secretion Signal
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 244
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A pGAP promoter is present on the pSB1C3 vector before the construction. It makes genome recombination easier in Pichia pastoris genome.
Source
The peptides DNA sequence has been obtained by reverse translate the amino acid sequence of the Leucrocin I proposed by Pata et al., 2011. Pata et al., 2011 had determinated the amino acid sequence by mass spectrometry analysis.
Pata, S., Yaraksa, N., Daduang, S., Temsiripong, Y., Svasti, J., Araki, T. and Thammasirirak, S. (2011). Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts. Developmental & Comparative Immunology, 35(5), pp.545-553.
Part:BBa_K1800001: Alpha-Factor Secretion Signal