Regulatory

Part:BBa_K2255004

Designed by: Camille Garcia   Group: iGEM17_Aix-Marseille   (2017-08-28)
Revision as of 22:54, 14 October 2017 by Kamy (Talk | contribs) (→‎Usage and Biology)


Xylella fastidiosa constitutive promoter + strong RBS

This sequence is a strong constitutive promoter with a strong RBS designed for Xylella fastidiosa.

It is the upstream sequence of a putative highly transcribed gene from Xylella fastidiosa 9a5c, found by analyzing the Best Bidirectional Hits (BBH) between Escherichia coli str. K-12 substr. MG1655 genes and Xylella fastidiosa 9a5c.

Usage and Biology

Fluorence levels of Escherichia coli DH5α bacteria transformed with pSB1C3 that contain sfGFP combined with various promoter and RBS.


This part enable us to produce in high quantities proteins in Xylella fastidiosa. It was engineered for the phage-like particle design of our project. Check out our [http://2017.igem.org/Team:Aix-Marseille/M13_Design design page] for more informations.

To evaluate the activity of the promoter and RBS, sfGFP protein was expressed in Escherichia coli DH5α bacteria and it fluorescence properties was studied. We compared our promoter and RBS to a well studied biobrick BBa_K608002 which is an E. coli strong promoter and RBS.

We used [http://2017.igem.org/Competition/InterLab_Study/Plate_Reader InterLab protocol] and negative control to test the fluorescence properties of these promoters and RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None