Coding
cNLS

Part:BBa_K2429124

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-12)
Revision as of 19:32, 13 October 2017 by Nmyrie (Talk | contribs)


Nuclear Localization Sequence (beginning)

This part codes for a peptide sequence that, when placed upstream of a gene sequence, codes for a peptide that indicates that the remaining portion of the translated protein will remain in the cell (specifically in the nucleus) rather then get excreted by the cell. The translated amino acid sequence of the NLS includes PKKKRRV and is classified as a classic nuclear localization sequence (cNLS). Such sequences are considered the best characterized transport signal [1].


[1] Lange, et al. "Classical Nuclear Localization Signals: Definition, Function, and Interaction with Importin α*"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 22
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/localization
Parameters
signalling_molecule