Coding

Part:BBa_K2324001:Design

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-11)
Revision as of 12:32, 11 October 2017 by Registry (Talk | contribs)

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FimH+sfGFP at residue 225


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation of CDS.


Source

All the components of this part are from the iGEM registry originally, but the CDS is codon optimised for expression in E.coli . The FimH sequence we used is part BBa_K1850001, the T7 promoter source was *INSERT HERE* and the GFP sequence was from part BBa_I746916.

References