Composite

Part:BBa_K2229300

Designed by: Catherine Yeh   Group: iGEM17_TAS_Taipei   (2017-09-26)
Revision as of 07:18, 26 September 2017 by Registry (Talk | contribs)

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Dual Expressing Construct of CsgD and OmpR234

The full construct that places a strong Promoter/RBS (BBa_K880005) in front of csgD (BBa_K805015), a transcriptional regulator of curli fimbriae and biofilm production. A Biobrick RBS (BBa_B0034) is inserted in front of the second protein OmpR234 (BBa_k342003), which promotes curli production by activating csgD. Both csgD and OmpR234 are acquired from the iGEM distribution kit, curli production is thus maximized through dual expression of the two proteins in Escherichia Coli. A double terminator (BBa_B0015) was added at the end to stop transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 877
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None