Measurement

Part:BBa_K2406053:Experience

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-09-29)
Revision as of 12:08, 7 October 2017 by JackSuitor (Talk | contribs)


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Applications of BBa_K2406053

This part is used to assay activity of Vika recombinase, whose expression is induced by treating cells with IPTG.

User Reviews

UNIQ76ab98a11e20b9ee-partinfo-00000000-QINU UNIQ76ab98a11e20b9ee-partinfo-00000001-QINU Construct is shown to work through experiment where cells were treated with IPTG and RFP fluorescence was measured. Control cells were not treated with IPTG. Results clearly show RFP is only expressed in presence of IPTG and thus when Vika recombinase is expressed. This confirms function of Vika recombinase, T7-LacO promoter, and Vox target sites. 200px|thumb|left|Graph showing expression of RFP when cells with measurement construct are induced with IPTG compared to uninduced cells.