Measurement
Part:BBa_K2406051
Designed by: Jack T. Suitor Group: iGEM17_Edinburgh_UG (2017-09-29)
Rox-Term-Rox Measurement construct.
Rox-Term-Rox construct inserted in BBa_J04450 part. If Dre recombinase is present it will cause recombination between the two rox sites, excising the terminator and allowing expression of the RFP protein. Therefore, this plasmid can be used as an assay for determining levels of Dre recombinase activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 876
Illegal AgeI site found at 988 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |