Coding

Part:BBa_K2255006

Designed by: Hussein Daher   Group: iGEM17_Aix-Marseille   (2017-08-28)
Revision as of 16:13, 29 September 2017 by Marlon (Talk | contribs)


EPS-Depolymerase (Rfc25)

Putative tail fiber protein/putative EPS Depolymerase (DIBBI_029) is an enzyme of 852 amino acids from the phage vB_XveM_DIBBI which contains two functions, one structural and one pectin lyase. Xanthomonas is a close relative of Xylella fastidiosa.

This part was designed with the Freiburg (Rfc25) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which ease the assembly of multiple protein domains.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None