Regulatory
WRKY30

Part:BBa_K2404005:Design

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-09-22)
Revision as of 17:27, 22 September 2017 by Registry (Talk | contribs)

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WRKY30 - a promoter induced by the presence of cellulose-derived oligomers


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 122
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 122
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 477
    Illegal BsaI.rc site found at 502


Design Notes

To isolate this promoter we needed to create a gene construct with the WRKY30 as the promoter element. We used the Golden Gate cloning system to do this, and so needed to isolate upstream restriction enzyme recognition sites (i.e. Type IIS restriction endonucleases) that are suitable for Golden Gate reactions. This consideration was taken into account when designing primers to isolate this part.


Source

This part comes from Arabidopsis thaliana and was isolated using specific primers.

References