Coding
TSHHantag
Part:BBa_K2404001:Design
Designed by: Ryan Coates Group: iGEM17_Cardiff_Wales (2017-09-22)
A Thyroid Stimulating Hormone antagonist with His-Tags
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 540
Illegal PstI site found at 582 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 540
Illegal PstI site found at 582 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 795
Illegal XhoI site found at 607 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 540
Illegal PstI site found at 582 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 540
Illegal PstI site found at 582 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 827
Design Notes
To produce this protein we needed to create a gene construct with the TSH antagonist (with His tags) as the coding sequence. We used the Golden Gate cloning system to do this, and so needed to create upstream restriction enzyme recognition sites (i.e. Type IIS restriction endonucleases) that are suitable for Golden Gate reactions.
Source
This modified protein was discovered in a paper by Fares et al in 2001, but without the His-tags. It was ordered as a g-block.