Composite

Part:BBa_K2443004

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-05)
Revision as of 17:58, 5 October 2017 by Graeme.glaister (Talk | contribs)


CysRS optimized for expression in E. coli

Cysteine tRNA synthetase responsible for attaching cysteine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 835
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752


[edit]
Categories
//collections/cell-free_tx-tl
Parameters
None