Coding

Part:BBa_K2443040:Design

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-04)
Revision as of 20:04, 4 October 2017 by Graeme.glaister (Talk | contribs) (→‎Source)


Ala RS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 464
    Illegal BglII site found at 671
    Illegal BamHI site found at 637
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1075
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2475
    Illegal BsaI site found at 2652
    Illegal BsaI.rc site found at 1024
    Illegal SapI site found at 1759


Design Notes

Codon optimized for Ecoli. All biobrick restriction sites removed



Source

http://www.uniprot.org/uniprot/P00957

References