Composite

Part:BBa_K2206009:Design

Designed by: Sammy Lovat   Group: iGEM17_CLSB-UK   (2017-10-02)
Revision as of 20:39, 2 October 2017 by Registry (Talk | contribs)

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Toehold switch for hsa-mir-27b-3p with luciferase and inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2096


Design Notes

We ensured that there are no stop codons between the start codon and the protein coding sequenc


Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.

References