Composite

Part:BBa_K2206003

Designed by: Sammy Lovat   Group: iGEM17_CLSB-UK   (2017-10-02)
Revision as of 19:30, 2 October 2017 by Registry (Talk | contribs)

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Toehold switch for hsa-miR-27b-3p with GFPmut3b

Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, resulting in translation of the protein coding region. This part codes for a toehold switch that contains a region that is complementary to the micro-RNA hsa-mir-27b-3p (the trigger RNA). The toehold switch is activated by hsa-mir-27b-3p and regulates production of GFPmut3b. Fluorescence intensity from GFP is proportional to the number of toehold switches activated, thus indicating the levels of hsa-mir-27b-3p present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 758


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Categories
Parameters
None