Composite
Part:BBa_K2379007
Designed by: Ramit B Group: iGEM17_SVCE_CHENNAI (2017-10-01)
pH sensitive system with RNA pHmeter
The RNA pHmeter with biobrick scar site(BBa_K2379002) is expressed under the regulation of the pLAC promoter(BBa_R0011). Following the RNA pHmeter, the construct contains GFP (BBa_E0040) as the reporter which is followed by the double terminator (BBa_B0014). GFP will get expressed only under alkaline conditions
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 181
Illegal BsaI.rc site found at 916
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Categories
Parameters
None |